aavrg- hsyn- egfp Search Results


avvrg hsyn dio egfp  (Addgene inc)
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Avvrg Hsyn Dio Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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gfp  (Addgene inc)
96
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aavrg-+hsyn-+egfp/pAAV-hSyn-EGFP+(Plasmid+%2350465)/pm36070750-417-10-12
Average 96 stars, based on 1 article reviews
gfp - by Bioz Stars, 2026-10
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aavrg hsyn hi egfp cre wpre sv40  (Addgene inc)
96
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Aavrg Hsyn Hi Egfp Cre Wpre Sv40, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aavrg-+hsyn-+egfp/pENN%2EAAV%2EhSyn%2EHI%2EeGFP-Cre%2EWPRE%2ESV40+(Plasmid+%23105540)/pmc11473139-19-0-6
Average 96 stars, based on 1 article reviews
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aav retrograde hsyn egfp  (Addgene inc)
94
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Aav Retrograde Hsyn Egfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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penn aavrg hsyn hi egfp cre wpre sv40 addgene 105540  (Addgene inc)
94
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Penn Aavrg Hsyn Hi Egfp Cre Wpre Sv40 Addgene 105540, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aavrg-+hsyn-+egfp/pAAV-EF1a-Cre+(Plasmid+%2355636)/pmc11025583-223-10-15
Average 94 stars, based on 1 article reviews
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aavrg hsyn jaws kgc egfp er2 wpre hghp a  (Addgene inc)
93
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Aavrg Hsyn Jaws Kgc Egfp Er2 Wpre Hghp A, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid rrid addgene 55632 aavrg hsyn hi egfp cre addgene  (Addgene inc)
96
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Plasmid Rrid Addgene 55632 Aavrg Hsyn Hi Egfp Cre Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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virus encoding cre recombinase  (Addgene inc)
96
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
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Average 96 stars, based on 1 article reviews
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raav2 retro hsyn mcherry  (Addgene inc)
97
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Raav2 Retro Hsyn Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aavrg hsyn dio mcherry  (Addgene inc)
96
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Aavrg Hsyn Dio Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aavrg hsyn dio hm4d gi mcherry  (Addgene inc)
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery <t>of</t> <t>retrograde</t> AAVs (retroAAV-hsyn; <t>GFP-Cre)</t> to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).
Aavrg Hsyn Dio Hm4d Gi Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery of retrograde AAVs (retroAAV-hsyn; GFP-Cre) to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).

Journal: Neuron

Article Title: NMDAR-dependent presynaptic homeostasis in adult hippocampus: Synapse growth and cross-modal inhibitory plasticity.

doi: 10.1016/j.neuron.2022.08.014

Figure Lengend Snippet: Figure 4. Genetic depletion of GluA1, GluA2, and GluA3 engages persistent synaptic compensation (A) Representative PCR-based genotyping results for triple heterozygous GRIA1, 2, 3 floxed mice. (B) Delivery of retrograde AAVs (retroAAV-hsyn; GFP-Cre) to the dorsal subiculum. (C) Images 4 weeks after virus injection. Slices immunolabeled anti-GFP (green) and neurons (NeuN; red). Nuclei are DAPI (blue). Scale bars are 500 mm (left and top right) and 50 mm (bottom right). (D–F) Representative traces (D), average amplitude (E), and frequency (F) of sEPSCs recorded in GFP-Cre+ cells (red) and GFP controls (green). (G) Representative traces and amplitudes of Qepsc recorded in 0.5 mM [Ca2+]e. (H) Success rates (Ps) of synaptic events for experiments shown in (G), (I), and (J). (I) Calcium input/output plots (transition from 0.5 mM to 2.5 mM [Ca2+]e). (J) Single experiments (light markers) and means (dark markers). Qepsc were determined for each cell during 10 min in low (0.5 mM) calcium. Max EPSC is the average plateau from calcium input/output. Data fit with a Power function. (K) Average of evoked EPSC amplitudes, including failures, at indicated [Ca2+]e. (L) Representative traces and quantification of average EPSCs (± SEM), normalized to EPSC1 (20 Hz). (M and N) PPRs (M) and the average of the last 5 EPSCs/1st (N). (O) Representative traces of AMPAR- and NMDAR-mediated EPSCs (left) and AMPAR/NMDAR ratios (right). n = # of cells, is shown in brackets, and is obtained from at least 3 different mice. ns p > 0.05; *p < 0.05; **p < 0.01; ***p < 0.001, Student’s two-tailed t test (E–H, J, K, M, and N), one-way ANOVA, and Dunnett’s multiple comparisons test, two-way repeated measures ANOVA (I and L).

Article Snippet: Retrograde serotype AAVs expressing GFP-Cre (pENN.AAVrg.hSyn.HI.eGFP-Cre.WPRE.SV40; Addgene #105540-AAVrg) or only GFP (pAAVrg-hSyn-EGFP; Addgene #50465-AAVrg) were injected into the following coordinates: X (posterior from bregma) 2.1mm, Y (lateral from bregma) ± 0.6mm, Z (ventral from pia) 1.55mm.

Techniques: Virus, Injection, Immunolabeling, Two Tailed Test